DNA Interactive Virtual Lab
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DNA Interactive / instructions
Directions: Today, you will be using the Internet to test and improve you understanding of DNA. Carefully follow the instructions below.
NOTE: shared work will result in a shared grade, split between ALL those involved in copying.
1. DNA Extraction virtual lab
- Go to http://learn.genetics.utah.edu/content/labs/extraction/
- Click on “Start Lab”
- Click “next” after reading the text on each page and answering the corresponding questions on your worksheet, under “DNA Extraction”
2. Gel Electrophoresis virtual lab
- Go to http://learn.genetics.utah.edu/content/labs/gel/
- Click on “Forward”
- Click “Forward” after reading the text on each page and answering the corresponding questions on your worksheet, under, under “Electrophoresis”
3. Transcription & Translation
- Go to https://learn.genetics.utah.edu/content/basics/txtl/
- Click on the button that says “Choose a Gene to Begin”
- Use the keyboard to type in the complementary base pairs to make an mRNA strand.
- Translate the mRNA strand you made into amino acids. Pay close attention to the “Universal Genetic Code” chart – all RNA messages must begin with a “Start” codon and end with a “Stop” codon, so you will have to shift the reading frame accordingly.
- Answer the questions on your worksheet, under “Transcription & Translation”.
4. DNA Replication
- Go to https://educationalgames.nobelprize.org/educational/medicine/dna_double_helix/
- Click on “Play” arrow
- Click through the information in the beginning to get to the game – you don’t need to read this stuff.
- Play all 3 rounds of the game. When the game is over, try to select the correct organism that matches each DNA strand you replicate.
5. If you finish early, check out http://www.dnai.org/a/index.html
- Choose one of the four modules (“Finding the Structure”; “Copying the Code”;
“Reading the Code”; and/or “Controlling the Code”)
- At the top right, click “Putting it together”
- Click on the “Interactive” icon to play
- Try each of the other modules until the bell rings
DNA Interactive \ worksheet
Directions: Answer the questions on this worksheet. Complete each section by following the instructions on the first page.
DNA EXTRACTION
- What are three reasons why we would need to extract DNA?
- Why does the DNA need to be extracted from a cell before it can be analyzed?
- About how much DNA is inside every cell’s nucleus?
GEL ELECTROPHORESIS
- What is electrophoresis used for?
- Why is “gel” used?
- What makes the DNA move?
- Which strands move father? Why do you think they are able to do this?
- What is a buffer? Why is it used in electrophoresis?
- Why it is important to put the black (negative) cord closest to the wells where the negatively
charged DNA was injected?
TRANSCRIPTION & TRANSLATION
- During transcription mRNA is translated into mRNA. Write out the complementary mRNA strand to the DNA strand below.
5’AAATGCTATGGCCTCTAATTTGCTGATATCAT3’
- Why do you think it is important for there to be designated “Start” and “Stop” codons?
3. Give the complement of the 3-letter mRNA code to make the correct amino acid.
UCA ___________
GUU ___________
CAG ___________
GAU ___________
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